Purification and characterization of 6-PGD from quail liver
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Purification and characterization of 6-PGD from quail liver
DE HC NWISBN: 9783659800009 bzw. 3659800007, in Deutsch, LAP Lambert Academic Publishing, gebundenes Buch, neu.
Lieferung aus: Deutschland, Versandkostenfrei innerhalb von Deutschland.
In this study, 6-phosphogluconate dehydrogenase (6PGD) enzyme was purified from the quail liver by using ammonium sulphate precipitation, and 2´, 5´-ADP Sepharose 4B affinity chromatography methods, with specific activities 14.6 EU/mg, 89% yield and 112 purification fold. Enzyme activity was measured spectrophotometrically at 340 nm. SDS-PAGE checked the purity of the enzyme. The molecular weight of purified enzyme by the SDS-PAGE method was calculated to be 44 KDa. Protein determination was In this study, 6-phosphogluconate dehydrogenase (6PGD) enzyme was purified from the quail liver by using ammonium sulphate precipitation, and 2´, 5´-ADP Sepharose 4B affinity chromatography methods, with specific activities 14.6 EU/mg, 89% yield and 112 purification fold. Enzyme activity was measured spectrophotometrically at 340 nm. SDS-PAGE checked the purity of the enzyme. The molecular weight of purified enzyme by the SDS-PAGE method was calculated to be 44 KDa. Protein determination was performed by the Bradford method. For the enzyme optimal pH (8.0), stable pH (8.0), optimum ionic strength (600 mM in Tris-HCl) optimal temperature (60°C) were determined. In addition KM and Vmax values for 6-phosphogluconate (6-PGA) and NADP+ were calculated as 0.058 and 0.0015 mM; 0.0078 and 0.057 EU/ml respectively. Versandfertig in 3-5 Tagen Lieferzeit 1-2 Werktage.
In this study, 6-phosphogluconate dehydrogenase (6PGD) enzyme was purified from the quail liver by using ammonium sulphate precipitation, and 2´, 5´-ADP Sepharose 4B affinity chromatography methods, with specific activities 14.6 EU/mg, 89% yield and 112 purification fold. Enzyme activity was measured spectrophotometrically at 340 nm. SDS-PAGE checked the purity of the enzyme. The molecular weight of purified enzyme by the SDS-PAGE method was calculated to be 44 KDa. Protein determination was In this study, 6-phosphogluconate dehydrogenase (6PGD) enzyme was purified from the quail liver by using ammonium sulphate precipitation, and 2´, 5´-ADP Sepharose 4B affinity chromatography methods, with specific activities 14.6 EU/mg, 89% yield and 112 purification fold. Enzyme activity was measured spectrophotometrically at 340 nm. SDS-PAGE checked the purity of the enzyme. The molecular weight of purified enzyme by the SDS-PAGE method was calculated to be 44 KDa. Protein determination was performed by the Bradford method. For the enzyme optimal pH (8.0), stable pH (8.0), optimum ionic strength (600 mM in Tris-HCl) optimal temperature (60°C) were determined. In addition KM and Vmax values for 6-phosphogluconate (6-PGA) and NADP+ were calculated as 0.058 and 0.0015 mM; 0.0078 and 0.057 EU/ml respectively. Versandfertig in 3-5 Tagen Lieferzeit 1-2 Werktage.
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Purification and characterization of 6-PGD from quail liver (2018)
EN PB NWISBN: 9783659800009 bzw. 3659800007, in Englisch, 56 Seiten, LAP Lambert Academic Publishing, Taschenbuch, neu.
Lieferung aus: Deutschland, Versandfertig in 3 - 4 Werktagen, Versandkostenfrei. Tatsächliche Versandkosten können abweichen.
Von Händler/Antiquariat, LitoA Buch- und Medienhandel.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
Von Händler/Antiquariat, LitoA Buch- und Medienhandel.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
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