Creation of a DNA aptamer biosensor for the detection of heart failure
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1
Creation of a DNA aptamer biosensor for the detection of heart failure
DE PB NW
ISBN: 9783659829239 bzw. 3659829234, in Deutsch, LAP Lambert Academic Publishing, Taschenbuch, neu.
Lieferung aus: Deutschland, Tbaħħir liberu.
Creation of a DNA aptamer biosensor for the detection of heart failure: Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles. Englisch, Taschenbuch.
Creation of a DNA aptamer biosensor for the detection of heart failure: Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles. Englisch, Taschenbuch.
2
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Creation of a DNA aptamer biosensor for the detection of heart failure
EN PB NW
ISBN: 9783659829239 bzw. 3659829234, in Englisch, LAP Lambert Academic Publishing, Taschenbuch, neu.
Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles.
3
Creation of a DNA aptamer biosensor for the detection of heart failure
~EN NW AB
ISBN: 9783659829239 bzw. 3659829234, vermutlich in Englisch, neu, Hörbuch.
Lieferung aus: Deutschland, Lieferzeit: 5 Tage.
Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles.
Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles.
4
Creation of a DNA aptamer biosensor for the detection of heart failure (2016)
EN PB NW
ISBN: 9783659829239 bzw. 3659829234, in Englisch, 332 Seiten, LAP LAMBERT Academic Publishing, Taschenbuch, neu.
Lieferung aus: Vereinigte Staaten von Amerika, Usually ships in 24 hours.
Von Händler/Antiquariat, Amazon.com.
Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles. Paperback, It-tikketta: LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Grupp ta ' prodott: Book, Ippubblikata: 2016-02-03, Data tal-ħruġ: 2016-02-03, Studio: LAP LAMBERT Academic Publishing.
Von Händler/Antiquariat, Amazon.com.
Aptamers are small nucleic acid molecules, which are used to bind specific targets in ways similar to antibodies. They offer advantages in terms of cost and reproducibility of production, stability and capability for biochemical modifications. This work focused on discovering a new DNA aptamer biosensor for brain natriuretic peptide (BNP), a biomarker of the early stages of heart failure. A library of random DNA sequences was generated by the polymerase chain reaction and evaluated for specific and sensitive binding to BNP using an in vitro selection technique known as systematic evolution of ligands by exponential enrichment (SELEX). After 15 SELEX cycles using BNP immobilised on an affinity chromatography column, selected aptamers were subcloned and sequenced. The aptamer exhibiting the best specificity towards BNP was shown to adopt a G-quadruplex structure of the anti-parallel type. This aptamer was used to devise an enzyme-linked oligonucleotide assay using surface enhanced Raman spectroscopy allowing the detection of BNP down to the picomolar range. In addition, a quick colorimetric assay was developed on the concept of target-mediated aggregation of gold nanoparticles. Paperback, It-tikketta: LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Grupp ta ' prodott: Book, Ippubblikata: 2016-02-03, Data tal-ħruġ: 2016-02-03, Studio: LAP LAMBERT Academic Publishing.
5
Creation of a DNA aptamer biosensor for the detection of heart failure
~EN PB NW
ISBN: 3659829234 bzw. 9783659829239, vermutlich in Englisch, LAP Lambert Academic Publishing, Taschenbuch, neu.
Creation of a DNA aptamer biosensor for the detection of heart failure ab 63.9 € als Taschenbuch: . Aus dem Bereich: Bücher, Wissenschaft, Biologie,.
7
Creation of a DNA aptamer biosensor for the detection of heart failure als von
DE HC NW
ISBN: 9783659829239 bzw. 3659829234, in Deutsch, LAP Lambert Academic Publishing, gebundenes Buch, neu.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
8
Creation of a DNA aptamer biosensor for the detection of heart failure (2016)
EN PB NW
ISBN: 9783659829239 bzw. 3659829234, in Englisch, 332 Seiten, LAP LAMBERT Academic Publishing, Taschenbuch, neu.
Lieferung aus: Vereinigtes Königreich Großbritannien und Nordirland, Usually dispatched within 24 hours.
Von Händler/Antiquariat, Amazon.co.uk.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
Von Händler/Antiquariat, Amazon.co.uk.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
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