The Role of AUF1 in AU-rich interleukin-6 mRNA regulation
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The Role of AUF1 in AU-rich interleukin-6 mRNA regulation (2016)
DE PB NW RPISBN: 9783659834844 bzw. 365983484X, in Deutsch, LAP Lambert Academic Publishing Mrz 2016, Taschenbuch, neu, Nachdruck.
Lieferung aus: Deutschland, Versandkostenfrei.
Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, Germany.
This item is printed on demand - Print on Demand Neuware - The AU-rich elements (AREs) consisting of repeated AUUUA motifs confer rapid degradation to many cellular mRNAs when present in the 3' untranslated region (3'UTR). We have studied the instability of interleukin-6 mRNA by grafting its 3'UTR to a stable green fluorescent protein mRNA. Subsequent scanning mutagenesis identified two conserved elements, which taken together account for most of the instability. Destabilization of ARE-containing mRNAs is thought to involve ARE-binding proteins such as AUF1. We tested whether AUF1 binding to interleukin-6 mRNA correlates with decreased mRNA stability. Overexpression of myc-tagged p37AUF1 and p42AUF1 as well as suppression of all four AUF1 isoforms by RNA interference stabilized the interleukin-6 mRNA. Furthermore, the interleukin-6 mRNA co-immunoprecipitated specifically with myc-tagged p37AUF1 and p42AUF1 in cell extracts. These results indicate that AUF1 binds to the AU-rich element in vivo and promotes interleukin-6 mRNA degradation. The combination of mRNA co-immunoprecipitation with microarray technology revealed that at least 500 cellular mRNAs associate with AUF1. 104 pp. Englisch.
Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, Germany.
This item is printed on demand - Print on Demand Neuware - The AU-rich elements (AREs) consisting of repeated AUUUA motifs confer rapid degradation to many cellular mRNAs when present in the 3' untranslated region (3'UTR). We have studied the instability of interleukin-6 mRNA by grafting its 3'UTR to a stable green fluorescent protein mRNA. Subsequent scanning mutagenesis identified two conserved elements, which taken together account for most of the instability. Destabilization of ARE-containing mRNAs is thought to involve ARE-binding proteins such as AUF1. We tested whether AUF1 binding to interleukin-6 mRNA correlates with decreased mRNA stability. Overexpression of myc-tagged p37AUF1 and p42AUF1 as well as suppression of all four AUF1 isoforms by RNA interference stabilized the interleukin-6 mRNA. Furthermore, the interleukin-6 mRNA co-immunoprecipitated specifically with myc-tagged p37AUF1 and p42AUF1 in cell extracts. These results indicate that AUF1 binds to the AU-rich element in vivo and promotes interleukin-6 mRNA degradation. The combination of mRNA co-immunoprecipitation with microarray technology revealed that at least 500 cellular mRNAs associate with AUF1. 104 pp. Englisch.
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The Role of AUF1 in AU-rich interleukin-6 mRNA regulation (2016)
DE PB NW RPISBN: 9783659834844 bzw. 365983484X, in Deutsch, Lap Lambert Academic Publishing, Taschenbuch, neu, Nachdruck.
Von Händler/Antiquariat, English-Book-Service Mannheim [1048135], Mannheim, Germany.
This item is printed on demand for shipment within 3 working days.
This item is printed on demand for shipment within 3 working days.
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The Role of AUF1 in AU-rich interleukin-6 mRNA regulation
~EN PB NWISBN: 365983484X bzw. 9783659834844, vermutlich in Englisch, LAP Lambert Academic Publishing, Taschenbuch, neu.
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The Role of AUF1 in AU-rich interleukin-6 mRNA regulation (2016)
EN PB NWISBN: 9783659834844 bzw. 365983484X, in Englisch, 104 Seiten, LAP LAMBERT Academic Publishing, Taschenbuch, neu.
Lieferung aus: Deutschland, Auf Lager. Lieferung von Amazon, Versandkostenfrei.
Von Händler/Antiquariat, Amazon.de.
LAP LAMBERT Academic Publishing, Taschenbuch, Publiziert: 2016-02-29T00:00:01Z, Produktgruppe: Book.
Von Händler/Antiquariat, Amazon.de.
LAP LAMBERT Academic Publishing, Taschenbuch, Publiziert: 2016-02-29T00:00:01Z, Produktgruppe: Book.
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